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KMID : 0613820040140030417
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Journal of Life Science
2004 Volume.14 No. 3 p.417 ~ p.424
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Lectin Histochemistry of the Glycoconjugates in the Esophageal Mucous Cells of Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus and Takifugu pardalis
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Jeong Kil-Nam
Lee Eung-Hee
Jo Gi-Jin
Jung Kwun-Soon
Jo Un-Bock
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Abstract
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This study attempts to investigate lectin binding patterns of the glycoconjugates of the esophageal mucous cells in four teleostean speceis, i. e., Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus and Takifugu pardalis. To investigate glycoconjugates of esophageal mucous cells, nine biotinylated lectins (DBA, SBA, PNA, BSL-1, RCA-1, sWGA, UEA-1, LCA and Con A) were applied with ABC method. Esophageal mucous cells of Sebastes schlegeli and Halichoeres poecilopterus were mixed with large, medium sized and small mucous cells. But these cells of the other species only were mixed with medium sized and small mucous cells. The lectin binding pattern of esophageal mucous cells depends on the species; Sebastes schlegeli was stained with DBA, SBA, BSL-1, RCA-1 and sWGA, Halichoeres poecilopterus with DBA, SBA, PNA and sWGA, Bryzoichthys lysimus with SBA and sWGA, Takifugu pardalis with all lectins except DBA, LCA and Con A, respectively. All the mucous cells of Sebastes schlegeli were stained with DBA, SBA and sWGA, while small mucous cells with BSL-1 besides these lectins. In Halichoeres poecilopterus, the large mucous cells reacted with PNA, medium sized mucous cells with DBA, SBA and sWGA, and small mucous cells with DBA and SBA, respectively. Medium sized mucous cells of Bryzoichthys lysimus were stained with sWGA, and small mucous cells with SBA and sWGA. In Takifugu pardalis, all mucous cells reacted with SBA, PNA and RCA-1, but medium sized mucous cells with sWGA and UEA-1 besides these lectins. Especially DBA and SBA lectins showed a strong binding to all mucous cells of Sebastes schlegeli. In Halichoeres poecilopterus, PNA binding were notable in large mucous cells, and SBA binding in medium sized and small cells, respectively. However, SBA, PNA, sWGA and UEA-1 lectins of Takifugu pardalis showed a strong binding to medium sized mucous cells, but RCA-1 binding which small mucous cells were notable
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KEYWORD
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Esophageal mucous cells, Lectin, Fishes
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